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Cbda synthase

30 Sep 2019 Cannabidiolic synthase (CBDA synthase) and tetrahydrocannabinolic acid synthase (THCA synthase) catalyze the oxidative cyclization of  12 Nov 2019 With this aim, this paper reports sequencing results for tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase  17 Jul 2015 The enzymes that produce THCA or CBDA (THCA synthase and CBDA synthase, respectively) were believed to be two alleles of the same  6 Aug 2014 Studies on tetrahydrocannabinolic acid synthase that produces the Purification and characterization of cannabidiolic-acid synthase from  As an outer-group of a dendrogram, nucleotide sequences of genes encoding CBDA synthase of C. sativa (accession number AB292682.1) and berberine 

Cannabinoids biosynthesis using recombinant cannabinoid synthase

Sequence heterogeneity of cannabidiolic- and

Cannabidiolic acid | C22H30O4 - PubChem

CBDA synthase catalyzes the formation of CBDA from cannabinerolic acid as well as cannabigerolic acid, although the k cat for the former (0.03 s −1) is lower than that for the latter (0.19 s −1). Therefore, we conclude that CBDA is predominantly biosynthesized from cannabigerolic acid rather than cannabinerolic acid. Purification and characterization of cannabidiolic-acid synthase CBDA synthase catalyzes the formation of CBDA from cannabinerolic acid as well as cannabigerolic acid, although the kcat for the former (0.03 s-1) is lower than that for the latter (0.19 s-1). Therefore, we conclude that CBDA is predominantly biosynthesized from cannabigerolic acid rather than cannabinerolic acid. Cannabinoids biosynthesis using recombinant cannabinoid synthase The cannabinoid synthase enzymes Tetrahydrocannabinolic acid (THCA) synthase and Cannabidiolic acid (CBDA) synthase were recombinantly produced in yeast Pichia pastoris Mut+ strain. The coding regions of THCA synthase and CBDA synthase genes were codon optimized for Pichia expression. Both synthase genes were operably linked to the methanol

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US8884100B2 - Aromatic prenyltransferase from Cannabis - Google Nucleic acid molecules from Cannabis sativa (cannabis, hemp, marijuana) have been isolated and characterized, and encode polypeptides having aromatic prenyltransferase activity. Specifically, the enzyme, CsPT1, is a geranylpyrophosphate olivetolate geranyltransferase, active in the cannabinoid biosynthesis step of prenylation of olivetolic acid Why Cannabigerol, or CBG, Could Be the Next Lucrative Cannabinoid